Efficacy of Ethanol Extract of Sambiloto (Andrographis paniculata) Against Pneumonia-Causing Klebsiella pneumoniae
DOI:
https://doi.org/10.19184/jid.v26i1.4616Keywords:
Andrographis paniculate, Covid - 19, Klebsiella pneumoniae, pneumonia, traditional medicineAbstract
Klebsiella pneumoniae is one of the most common pathogens in nosocomial respiratory infections. Antibiotics are often used for the treatment of K. pneumoniae infections. Increased bacterial resistance to antibiotics is closely related to the success rate of infectious disease therapy. The use of plants as phytopharmacology is currently an alternative treatment. Some medicinal plants that have antibacterial and immunomodulatory activity are Sambiloto (Andrographis paniculata) with an active compound called andrographol. The purpose of this study was to provide a solution to the problem above by developing a phytopharmaceutical using bitter extract to inhibit the growth of K. pneumoniae bacteria. Two sputum samples from the laboratory confirmed Covid-19 patients with pneumonia infection at the Hospital. The sambiloto was extracted by using 96% ethanol. The extract was further analyzed for its antimicrobial activity and phytochemical compounds. There were two K. pneumoniae isolates, namely KpRNG and KpTRI. The active compounds were saponin and steroids. The antimicrobial activity of the ginger extract was measured with different concentrations of 25%, 50%, 75%, and 100%. Its inhibitory zone against K. pneumoniae was ranging from 5.8 to 7 mm or had a moderate inhibition category.
References
Ahmad, Q., Sabrina, T., Diba, M. F., Amalia, E., & Putra, R. A. (2021). Gambaran infeksi klebsiella pneumoniae penghasil extended- spectrum β -lactamase ( ESBL ) Pada Pasien Covid-19 Di RSUP DR. Mohammad Hoesin. JMJ, JAMHESIC, 186-198.
Alkandahri, M. Y., Buana, U., Karawang, P., Subarnas, A., Padjadjaran, U., Berbudi, A., & Padjadjaran, U. (2018). Review. Aktifitas Immunomodulator Tanaman Sambiloto Farmaka. Departemen Farmakologi Dan Toksikologi, Fakultamasi, Universitas Padjadjaran, September. https://doi.org/10.24198/jf.v16i3.14237
Anand, T., Virmani, N., Kumar, S., Mohanty, A. K., Pavulraj, S., Bera, B. C., Vaid, R. K., Ahlawat, U., & Tripathi, B. N. (2020). Phage therapy for treatment of virulent Klebsiella pneumoniae infection in a mouse model. Journal of Global Antimicrobial Resistance, 21, 34-41. https://doi.org/10.1016/j.jgar.2019.09.018
Chang, J. S., Wang, K. C., Yeh, C. F., Shieh, D. E., & Chiang, L. C. (2013). Fresh ginger (Zingiber officinale) has anti-viral activity against human respiratory syncytial virus in human respiratory tract cell lines. Journal of Ethnopharmacology, 145(1), 146–151. https://doi.org/10.1016/j.jep.2012.10.043
Doss, V. A., & Kalaichelvan, P. T. (2012). In vitro antimicrobial and antioxidant activity screening of andrographis paniculata leaf ethanolic extract in Tamilnadu. International Journal of Pharmacy and Pharmaceutical Sciences, 4(1), 227-229.
Harapan, I. K., Tahulending, A., & Tumbol, M. V. L. (2018). Karakteristik resistensi klebsiella pneumoniae yang resisten karbapenem pada beberapa rumah sakit di Indonesia dan pemeriksaan laboratorium. Prosiding Seminar Nasional Tahun 2018 Menuju Masyarakat Sehat, Berkarakter Dan Berprestasi, 1(3), 636–650.
Hardodianto, R., Putra, P., Widyaningrum, I., Fadli, M. Z., Hardodianto, R., Putra, P., Widyaningrum, I., & Fadli, M. Z. (2000). Jahe merah dan lengkuas merah the activity of a combination of ethyl acetate extract.
Huang, C., Tao, S., Yuan, J., & Li, X. (2022). Effect of sodium hypochlorite on biofilm of Klebsiella pneumoniae with different drug resistance. American Journal of Infection Control, 50(8), 922–928. https://doi.org/10.1016/j.ajic.2021.12.003
Komori, A., Abe, T., Kushimoto, S., Ogura, H., Shiraishi, A., Saitoh, D., Fujishima, S., Mayumi, T., Naito, T., Hifumi, T., Shiino, Y., Nakada, T. aki, Tarui, T., Otomo, Y., Okamoto, K., Umemura, Y., Kotani, J., Sakamoto, Y., Sasaki, J., … Mizushima, Y. (2020). Characteristics and outcomes of bacteremia among ICU-admitted patients with severe sepsis. Scientific Reports, 10(1), 1–8. https://doi.org/10.1038/s41598-020-59830-6
Nayak, B. K., Pavithera, S., & Nanda, A. (2015). Soxhlet extraction of leaf extracts of Andrographis paniculata and its antibacterial efficacy against few pathogenic bacterial strains. Der Pharmacia Lettre, 7(4), 258-261.
Nur Rachmani, E. P., Pramono, S., & Nugroho, A. E. (2018). Aktifitas Antioksidan fraksi flavonoid bebas andrografolid dari herba sambioloto (Andrographis paniculata). Jurnal Farmasi Medica/Pharmacy Medical Journal (PMJ), 1(2), 42-49. https://doi.org/10.35799/pmj.1.2.2018.21642
Patel Singh, S., Yaduvanshi, N., Sahu, C., Singh, S., & Agarwal, A. (2021). Epidemiology, Antimicrobial susceptibility patterns and outcomes of bacteremia in an Apex trauma center of a tertiary health care institute with special reference to Methicillin Re... International Journal of Medical Science and Current Research, 4(2), 435-443.
Priyani. (2020). Review : Manfaat tanaman sambiloto (. Jurnal Ilmu Kedokteran Dan Kesehatan, 7, 484-490.
Sah, S. K., Rasool, U., & Hemalatha, S. (2020). Andrographis paniculata extract inhibit growth, biofilm formation in multidrug resistant strains of Klebsiella pneumoniae. Journal of Traditional and Complementary Medicine, 10(6), 599-604. https://doi.org/10.1016/j.jtcme.2019.02.006
Shaikh, S., Fatima, J., Shakil, S., Rizvi, S. M. D., & Kamal, M. A. (2015). Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Sciences, 22(1), 90-101. https://doi.org/10.1016/j.sjbs.2014.08.002
Sholikhah, E. N. (2016). Indonesian medicinal plants as sources of secondary metabolites for pharmaceutical industry. Journal of Thee Medical Sciences (Berkala Ilmu Kedokteran), 48(04), 226-239. https://doi.org/10.19106/jmedsci004804201606
Silaban, H. (2021). The effect of various concentrations of ethanol extract of the leaves of paederia foetida L. on the growth of escherichia coli bacteria. Journal of Drug Delivery and Therapeutics, 11(6), 61–67. https://doi.org/10.22270/jddt.v11i6.5037
Stahlhut, S. G., Struve, C., Krogfelt, K. A., & Reisner, A. (2012). Biofilm formation of Klebsiella pneumoniae on urethral catheters requires either type 1 or type 3 fimbriae. FEMS Immunology and Medical Microbiology, 65(2), 350-359. https://doi.org/10.1111/j.1574-695X.2012.00965.x
Suryelita, S., Riga, R., Etika, S. B., Ulfah, M., & Artasasta, M. A. (2021). Antibacterial screening of endophytic fungus xylaria sp. Derived from andrographis paniculata (sambiloto). Open Access Macedonian Journal of Medical Sciences, 9, 971-975. https://doi.org/10.3889/oamjms.2021.7475
Utami, L. P., Tandean, P. G., & Liliawanti, L. (2020). Pengaruh pemberian ekstrak kencur (kaempferia galanga L.) terhadap peningkatan zona hambat pertumbuhan bakteri staphylococcus aureus. Jurnal Ilmiah Kedokteran Wijaya Kusuma, 9(2), 145. https://doi.org/10.30742/jikw.v9i2.883
Zhang, G., Jiang, C., Xie, N., Xu, Y., Liu, L., & Liu, N. (2019). Treatment with andrographolide sulfonate provides additional benefits to imipenem in a mouse model of Klebsiella pneumoniae pneumonia. Biomedicine and Pharmacotherapy, 117(March), 109065. https://doi.org/10.1016/j.biopha.2019.109065
