Journal of Agropharmacy

Daftar Isi

2024-03-01

Skrining Fitokimia dan Evaluasi Antibakteri Ekstrak NADES (Natural Deep Eutectic Solvents) Daun Iler (Coleus atropurpureus L. Benth) terhadap Bakteri Penyebab Folikulitis

Evi Umayah Ulfa — 2024-03-01

The iler plant (Coleus atropurpureus) can be used as an efficacious medicinal plant. The chemical contents of the iler plant include essential oils, alkaloid, polyphenols, tannins and flavonoids. This content can be used as an antibacterial. This study aims to determine the antibacterial activity of iler leaves (Coleus atropurpureus) against bacterial folliculitis (Staphylococcus
aureus). The NADES combination used is choline chloride:sorbitol with a ratio of 1:1, 2:1, and 3:1. The extraction method uses the UAE method and the antibacterial activity test uses the well diffusion method. The positive control used was Ciprofloxacin. The diameter of the inhibition zone for S. aureus bacteria is in a ratio of 1:1 (4,53 mm), 2:1 (6,29 mm), and 3:1 (8,12). The results of the phytochemical screening test obtained showed that the NADES extract of Iler leaves was positive for containing alkaloids, flavonoids, polyphenols and tannins.

Potensi Antimalaria Fraksi-Fraksi Daun Kembang Bulan (Tithonia diversifolia [Hemsley] A. Gray)

Nuri — 2024-03-01

Kembang bulan (Tithonia diversifolia [Hemsley] A. Gray) has been used in traditional medicine to treat malaria. Previous research results showed that the ethanol extract of kembang bulan leaves had antimalarial activity in vivo with an ED₅₀ of 113.39 mg/kgBW. Until now, the compounds with antimalarial activity in vivo are unknown. The initial stage that can be done to separate the compounds in the extract is fractionation. This study aims to separate the extract into fractions and test its antimalarial activity. Fractionation was carried out using a vacuum liquid chromatography method using a stationary
phase of silica gel GF₂₅₄ and a mobile phase of n-hexane, chloroform and methanol in a gradient. In vivo antimalarial activity was tested using the Peter test method. Fractionation produces 5 fractions, each yield is 1.9; 2.1; 17.6; 60.8, and 17.6% respectively for fractions 1-5. The results of the antimalarial activity test for fractions 1-5 with a dose of 2 mg/kgBW showed inhibition of the growth of Plasmodium berghei respectively at 52.3 ± 1.4; 83.5±1.4; 74.6±1.2; 69.5±0.9; 44.8 ± 0.3%. Fraction 2 with the greatest resistance was tested further and showed an ED₅₀ value of 0.52 mg/kgBW.

Analisis Minimalisasi Biaya Penggunaan Antiplatelet Pada Pasien Stroke Infark Rawat Inap

Ayssa Wicaksono — 2024-03-01

Antiplatelets are crucial medications in the treatment of ischemic stroke patients. The management of stroke cases in Indonesia incurs substantial costs, reaching Rp. 3.2 billion annually. This study conducted a simple pharmacoeconomic research using Cost Minimization Analysis (CMA) method, utilizing medical records and patient ﬁnancing data of ischemic stroke inpatients at RSUD Gambiran, Kediri City from January to December 2022. The study adopts a hospital perspective with the same outcome of patient recovery. Out of 659 stroke patients, 100 eligible participants, predominantly male aged 46-65 years with Diabetes Mellitus as the most common comorbidity, were included. Based on antiplatelet therapy proﬁles, the majority received a combination of clopidogrel and aspirin (65%), while 35% received clopidogrel monotherapy. The largest cost component was service cost amounting to Rp 2,898,606 (59.37%). According to CMA analysis, combination therapy showed cost-saving beneﬁts compared to monotherapy in drug and medical equipment, laboratory, service, and total overall costs. Thus, for ischemic stroke patients with the same outcome of recovery, combination therapy of clopidogrel and aspirin is
recommended over clopidogrel monotherapy.

Penentuan Kadar Flavonoid Total dan Klasifikasi Serbuk Daun Murbei (Morus alba L.) Berbeda Ketinggian Tempat Tanam

Abim Syaifullah — 2024-03-01

Mulberry (Morus alba L.) is a plant from the Moraceae family which has a high flavonoid content. This research aims to determine the ability of NIR spectroscopy and chemometrics to classify mulberry leaf powder based on different planting heights and determine the total flavonoid content using the Uv-Vis spectrophotometric method. The classification of mulberry leaf powder originating from the lowlands, midlands, and highlands provides valid results using NIR spectra and chemometric data from the LDA and SIMCA models with an accuracy value of 100%. The average results of determining total flavonoid content using Uv-Vis spectrophotometry showed that the highest levels were in the highlands, followed by the medium and lowlands with total flavonoid levels respectively, namely 6.453 ± 1.194 mg QE/g powder; 5.889 ± 0.944 mg QE/g powder; and 5.177 ± 0.998 mg QE/g powder. The results of the One Way ANOVA test showed that the total flavonoid content of mulberry leaf powder from different altitude areas did not have a significant difference.

Pembentukan kokristal Ketoprofen-Urea-NaCl untuk Peningkatan Sifat Kelarutan

Aisyah Prida Laily — 2024-03-01

Ketoprofen is a potent non-steroidal anti-inflammatory drug compound but is difficult to dissolve in water. This research aims to increase the solubility of ketoprofen through cocrystallization using a urea coformer with the addition of the inorganic salt NaCl. Cocrystallization was carried out using the solvent evaporation method using methanol. The characterization of the cocrystal results included PXRD, DSC, and FTIR. At the same time, the solubility evaluation was carried out using a solubility test in distilled water and a dissolution test in phosphate buffer media pH 7.4. The results showed that ketoprofen forms a cocrystal with the urea coformer, whereas adding NaCl produces a ketoprofen-urea-NaCl cocrystal with a lower crystallinity and melting point. The solubility of ketoprofen-urea-NaCl cocrystals in distilled water was 183.82 ± 2.84 mg/L, while ketoprofen-urea and ketoprofen cocrystals were 174.23 ± 6.83 and 171.12 ± 6.29 mg/L, respectively. The dissolution test in phosphate buffer medium pH 7.4 showed the dissolution rate of ketoprofen-urea-NaCl cocrystals > ketoprofen-urea cocrystals > ketoprofen.